diphtheria toxoid plasmid dt5 (Addgene inc)
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diphtheria toxoid plasmid dt5
Diphtheria Toxoid Plasmid Dt5, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet+22b+dt+51e+148k/pET-22b+DT+51E%2F148K+(Plasmid+%2311081)/us12516283-411-1-8
Average 94 stars, based on 11 article reviews
Diphtheria Toxoid Plasmid Dt5, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet+22b+dt+51e+148k/pET-22b+DT+51E%2F148K+(Plasmid+%2311081)/us12516283-411-1-8
Average 94 stars, based on 11 article reviews
diphtheria toxoid plasmid dt5 - by Bioz Stars,
2026-09
94/100 stars
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Polymerase Chain Reaction:Article Title: IgAim: cell surface Ig-aimed immune memory erasers for the therapy of autoimmune diseases and B leukemia Article Snippet: The anti-Phl p7 human monoclonal antibody expression vector was constructed by antibody light and heavy chain polymerase chain reaction (PCR) product into a BOS-promoter lentiviral expression vector, where the antibody light chain gene and heavy chain VDJ gene are derived from pVITRO1-102.1F10-IgE/lambda (Addgene #50365), membrane-type Cgamma1 gene from KARPAS-422 cDNA, and a furin T2A linker from oligos. .. The GGGS linker (SSGGGSSGGGS), the furin T2A linker (RRKRGSGLINEGRGSLLTCGDVEENPGP), and the Phl p7 gene were created by PCR amplification of overlapping oligos. Article Title: IgAim: cell surface Ig-Aimed Immune Memory erasers for the therapy of autoimmune diseases and B leukemia Article Snippet: The anti-Phl p7 human monoclonal antibody expression vector was constructed by antibody light and heavy chain polymerase chain reaction (PCR) product into a BOS-promoter lentiviral expression vector, where the antibody light chain gene and heavy chain VDJ gene are derived from pVITRO1-102.1F10IgE / lambda (Addgene #50365), membrane-type Cgamma1 gene from KARPAS-422 cDNA, and a furin T2A linker from oligos. .. The GGGS linker (SSGGGSSGGGS), the furin T2A linker (RRKRGSGLINEGRGSLLTCGDVEENPGP), and the Phl p7 gene were created by PCR amplification of overlapping oligos. Amplification:Article Title: IgAim: cell surface Ig-aimed immune memory erasers for the therapy of autoimmune diseases and B leukemia Article Snippet: The anti-Phl p7 human monoclonal antibody expression vector was constructed by antibody light and heavy chain polymerase chain reaction (PCR) product into a BOS-promoter lentiviral expression vector, where the antibody light chain gene and heavy chain VDJ gene are derived from pVITRO1-102.1F10-IgE/lambda (Addgene #50365), membrane-type Cgamma1 gene from KARPAS-422 cDNA, and a furin T2A linker from oligos. .. The GGGS linker (SSGGGSSGGGS), the furin T2A linker (RRKRGSGLINEGRGSLLTCGDVEENPGP), and the Phl p7 gene were created by PCR amplification of overlapping oligos. Article Title: IgAim: cell surface Ig-Aimed Immune Memory erasers for the therapy of autoimmune diseases and B leukemia Article Snippet: The anti-Phl p7 human monoclonal antibody expression vector was constructed by antibody light and heavy chain polymerase chain reaction (PCR) product into a BOS-promoter lentiviral expression vector, where the antibody light chain gene and heavy chain VDJ gene are derived from pVITRO1-102.1F10IgE / lambda (Addgene #50365), membrane-type Cgamma1 gene from KARPAS-422 cDNA, and a furin T2A linker from oligos. .. The GGGS linker (SSGGGSSGGGS), the furin T2A linker (RRKRGSGLINEGRGSLLTCGDVEENPGP), and the Phl p7 gene were created by PCR amplification of overlapping oligos. Article Title: Unconventional structure and mechanisms for membrane interaction and translocation of the NF-κB-targeting toxin AIP56 Article Snippet: .. DTR S406-S560 coding sequence (accession number WP_072564851.1 ) was then amplified from Article Title: Structural and functional characterization of the NF-κB-targeting toxin AIP56 from Photobacterium damselae subsp. piscicida reveals a novel mechanism for membrane interaction and translocation Article Snippet: .. DTR S406-S560 coding sequence (accession number WP_072564851.1) was then amplified from Article Title: Unconventional structure and mechanisms for membrane interaction and translocation of the NF-κB-targeting toxin AIP56. Article Snippet: .. DTRS406-S560 coding sequence (accession number WP_072564851.1) was then amplified from Plasmid Preparation:Article Title: IgAim: cell surface Ig-aimed immune memory erasers for the therapy of autoimmune diseases and B leukemia Article Snippet: The anti-Phl p7 human monoclonal antibody expression vector was constructed by antibody light and heavy chain polymerase chain reaction (PCR) product into a BOS-promoter lentiviral expression vector, where the antibody light chain gene and heavy chain VDJ gene are derived from pVITRO1-102.1F10-IgE/lambda (Addgene #50365), membrane-type Cgamma1 gene from KARPAS-422 cDNA, and a furin T2A linker from oligos. .. The GGGS linker (SSGGGSSGGGS), the furin T2A linker (RRKRGSGLINEGRGSLLTCGDVEENPGP), and the Phl p7 gene were created by PCR amplification of overlapping oligos. Article Title: IgAim: cell surface Ig-Aimed Immune Memory erasers for the therapy of autoimmune diseases and B leukemia Article Snippet: The anti-Phl p7 human monoclonal antibody expression vector was constructed by antibody light and heavy chain polymerase chain reaction (PCR) product into a BOS-promoter lentiviral expression vector, where the antibody light chain gene and heavy chain VDJ gene are derived from pVITRO1-102.1F10IgE / lambda (Addgene #50365), membrane-type Cgamma1 gene from KARPAS-422 cDNA, and a furin T2A linker from oligos. .. The GGGS linker (SSGGGSSGGGS), the furin T2A linker (RRKRGSGLINEGRGSLLTCGDVEENPGP), and the Phl p7 gene were created by PCR amplification of overlapping oligos. Article Title: Unconventional structure and mechanisms for membrane interaction and translocation of the NF-κB-targeting toxin AIP56 Article Snippet: .. DTR S406-S560 coding sequence (accession number WP_072564851.1 ) was then amplified from Article Title: Structural and functional characterization of the NF-κB-targeting toxin AIP56 from Photobacterium damselae subsp. piscicida reveals a novel mechanism for membrane interaction and translocation Article Snippet: .. DTR S406-S560 coding sequence (accession number WP_072564851.1) was then amplified from Article Title: Selective and Effective Cancer Treatments using Target‐Switchable Intracellular Bacterial Toxin Delivery Systems Article Snippet: Targeted cancer therapies have been extensively tested with the purpose to selectively suppress tumor growth and to avoid harming healthy tissue.. However, failure to escape endosomes upon receptor-mediated endocytosis is a major obstacle limiting the efficacy of targeted cancer therapeutics.. Here, novel target-switchable intracellular toxin delivery systems (TiTDS) are presented which use the catalytic and translocation domain of diphtheria toxin (dtA-T) as an intracellular toxin delivery platform and affibody molecules targeting human epidermal growth factor receptor 2 or epidermal growth factor receptor (HER2Afb or EGFRAfb) as target-specific ligands. Article Title: Unconventional structure and mechanisms for membrane interaction and translocation of the NF-κB-targeting toxin AIP56. Article Snippet: .. DTRS406-S560 coding sequence (accession number WP_072564851.1) was then amplified from Article Title: IgAim: cell surface Ig-aimed immune memory erasers for the therapy of autoimmune diseases and B leukemia Article Snippet: .. The control vector was constructed by introducing amino acid substitutions of E82K and K179E into Cloning:Article Title: IgAim: cell surface Ig-aimed immune memory erasers for the therapy of autoimmune diseases and B leukemia Article Snippet: The anti-Phl p7 human monoclonal antibody expression vector was constructed by antibody light and heavy chain polymerase chain reaction (PCR) product into a BOS-promoter lentiviral expression vector, where the antibody light chain gene and heavy chain VDJ gene are derived from pVITRO1-102.1F10-IgE/lambda (Addgene #50365), membrane-type Cgamma1 gene from KARPAS-422 cDNA, and a furin T2A linker from oligos. .. The GGGS linker (SSGGGSSGGGS), the furin T2A linker (RRKRGSGLINEGRGSLLTCGDVEENPGP), and the Phl p7 gene were created by PCR amplification of overlapping oligos. Article Title: IgAim: cell surface Ig-Aimed Immune Memory erasers for the therapy of autoimmune diseases and B leukemia Article Snippet: The anti-Phl p7 human monoclonal antibody expression vector was constructed by antibody light and heavy chain polymerase chain reaction (PCR) product into a BOS-promoter lentiviral expression vector, where the antibody light chain gene and heavy chain VDJ gene are derived from pVITRO1-102.1F10IgE / lambda (Addgene #50365), membrane-type Cgamma1 gene from KARPAS-422 cDNA, and a furin T2A linker from oligos. .. The GGGS linker (SSGGGSSGGGS), the furin T2A linker (RRKRGSGLINEGRGSLLTCGDVEENPGP), and the Phl p7 gene were created by PCR amplification of overlapping oligos. Article Title: Selective and Effective Cancer Treatments using Target‐Switchable Intracellular Bacterial Toxin Delivery Systems Article Snippet: Targeted cancer therapies have been extensively tested with the purpose to selectively suppress tumor growth and to avoid harming healthy tissue.. However, failure to escape endosomes upon receptor-mediated endocytosis is a major obstacle limiting the efficacy of targeted cancer therapeutics.. Here, novel target-switchable intracellular toxin delivery systems (TiTDS) are presented which use the catalytic and translocation domain of diphtheria toxin (dtA-T) as an intracellular toxin delivery platform and affibody molecules targeting human epidermal growth factor receptor 2 or epidermal growth factor receptor (HER2Afb or EGFRAfb) as target-specific ligands. Sequencing:Article Title: Unconventional structure and mechanisms for membrane interaction and translocation of the NF-κB-targeting toxin AIP56 Article Snippet: .. DTR S406-S560 coding sequence (accession number WP_072564851.1 ) was then amplified from Article Title: Structural and functional characterization of the NF-κB-targeting toxin AIP56 from Photobacterium damselae subsp. piscicida reveals a novel mechanism for membrane interaction and translocation Article Snippet: .. DTR S406-S560 coding sequence (accession number WP_072564851.1) was then amplified from Article Title: Unconventional structure and mechanisms for membrane interaction and translocation of the NF-κB-targeting toxin AIP56. Article Snippet: .. DTRS406-S560 coding sequence (accession number WP_072564851.1) was then amplified from Control:Article Title: IgAim: cell surface Ig-Aimed Immune Memory erasers for the therapy of autoimmune diseases and B leukemia Article Snippet: .. The control vector was constructed by introducing amino acid substitutions of E82K and K179E into Article Title: IgAim: cell surface Ig-aimed immune memory erasers for the therapy of autoimmune diseases and B leukemia Article Snippet: .. The control vector was constructed by introducing amino acid substitutions of E82K and K179E into Construct:Article Title: IgAim: cell surface Ig-Aimed Immune Memory erasers for the therapy of autoimmune diseases and B leukemia Article Snippet: .. The control vector was constructed by introducing amino acid substitutions of E82K and K179E into Article Title: IgAim: cell surface Ig-aimed immune memory erasers for the therapy of autoimmune diseases and B leukemia Article Snippet: .. The control vector was constructed by introducing amino acid substitutions of E82K and K179E into Binding Assay:Article Title: IgAim: cell surface Ig-Aimed Immune Memory erasers for the therapy of autoimmune diseases and B leukemia Article Snippet: .. The control vector was constructed by introducing amino acid substitutions of E82K and K179E into Article Title: IgAim: cell surface Ig-aimed immune memory erasers for the therapy of autoimmune diseases and B leukemia Article Snippet: .. The control vector was constructed by introducing amino acid substitutions of E82K and K179E into Purification:Article Title: Selective and Effective Cancer Treatments using Target‐Switchable Intracellular Bacterial Toxin Delivery Systems Article Snippet: Targeted cancer therapies have been extensively tested with the purpose to selectively suppress tumor growth and to avoid harming healthy tissue.. However, failure to escape endosomes upon receptor-mediated endocytosis is a major obstacle limiting the efficacy of targeted cancer therapeutics.. Here, novel target-switchable intracellular toxin delivery systems (TiTDS) are presented which use the catalytic and translocation domain of diphtheria toxin (dtA-T) as an intracellular toxin delivery platform and affibody molecules targeting human epidermal growth factor receptor 2 or epidermal growth factor receptor (HER2Afb or EGFRAfb) as target-specific ligands. |